The study of mechanobiology incorporates functional elements and processes of a cell that work in unison to measure, sense and respond to the extracellular environment. For example; cell movement enables mechanosensitive elements to detect external mechanical constraints, but cell movement can also act as a response to an external mechanical signal that is transduced through intracellular biomechanical signaling. With countless intracellular feedback mechanisms regulating each of the various functional elements, developing methods to study this field often requires new technologies and unique procedures. This topic will explore several of the methods commonly used in the field of mechanobiology.